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Image Search Results
Journal: Molecular Neurobiology
Article Title: Posttranscriptional Regulation of Gene Expression Participates in the Myelin Restoration in Mouse Models of Multiple Sclerosis: Antisense Modulation of HuR and HuD ELAV RNA Binding Protein
doi: 10.1007/s12035-023-03236-8
Figure Lengend Snippet: Effect of HuR silencing on myelination in SNI mice. A Silencing of HuR increased MBP, marker of myelin, in spinal cord of SNI mice (one-way ANOVA * p < 0.05 vs dODN ipsi) and lack of effect by HuD silencing. Representative blots are reported. The signal intensity was normalized to that of total protein. B Quantitative analysis of Luxol Fast Blue colorimetric assay and C representative image of spinal cord tissue of dODN, anti-HuR ASO, and anti-HuD ASO (white color corresponds to myelin, scale bar = 100 µm) (one-way ANOVA * p < 0.05 vs dODN ipsi). D Silencing of HuR and HuD did not alter the expression levels of S100 in sciatic nerve (one-way ANOVA, * p < 0.05 vs dODN contra). Representative blots are reported. The signal intensity was normalized to that of total protein. E Representative images of the expression of S100 (red) of ipsilateral side of sciatic nerve in SNI mice. Scale bar = 50 µm. Results are expressed as mean ± SEM. Data are the mean of five individual experiments
Article Snippet: Blots were incubated overnight at 4 °C with specific antibodies against IBA1 (1:1000; sc–32725; RRID:AB_667733), HuD (1: 1000; sc–48421; RRID:AB_627766); HuR (1:1000, sc–5261; RRID:AB_627770), MBP (1:1000 sc–271524; RRID:AB_10655672),
Techniques: Marker, Colorimetric Assay, Expressing